The Core facility laboratory is a nonprofit joint venture between the School of Medicine and University Hospital. It is directed by Dr. Marc Golightly. The Laboratory is part of the Clinical Immunology Laboratories and is housed in University Hospital. The laboratory is equipped with a Becton Dickinson FACSCAN analyzer(Argon laser), two FACS Caliburs (argon & red diode lasers) and a three laser FACS VANTAGE Cell Sorter (argon, Helium-neon, and UV lasers) with full supportive equipment. Five full time technologists staff it. (click here to visit our other Immunology laboratories and tutorials)
Core Facility Services
Consultative
Services - Experimental setup/review, analysis interpretation,
manuscript review
Rate
and Fee Structure - Rates for services /instruments/ analysis
& Billing information
Scheduling
Facility Time - Policies, Internet scheduling, phone scheduling
Links to other Flow
Cytometry Core Facilities
The Flow
Cytometry Crew
(L to R, Corinne, Nancy, Todd, Chris, Kerri)
In Memory of Christopher Pullis (1952-2004):
Christopher
was the supervisor of the Flow Cytometry Core Facility and the University
Hospital Clinical Flow Cytometry Laboratory. He gave 24 years ofextremely
dedicated service to Stony Brook University. His expertise and knowledge
was pivotal to the establishment, development, and maintenance of theFlow
Cytometry Facilities. While his extreme professionalism was just a given,
it was his warmth, compassion, and unforgettable sense of humor which
touched everyone around him. Our colleague and friend will be sorely missed.
A fund is set up for Christopher’s children. Those interested may call the Immunology Laboratory for details (444-3744).
- Free consultation to discuss the application of Flow Cytometry to the principal investigator's project or proposed use. All users are encouraged (required) to do this . This consultation often provides the PI alternatives, insures proper controls will be run, and often eliminates repeat studies due to technical inexperience with Flow Cytometry.
- Free review of data presentation for publication. It has been published that a large percentage of published Flow Cytometry data is presented wrong or erroneous conclusion have been made. This service seeks to eliminate errors in data presentation/conclusions thus increasing the chances for manuscript acceptance.
- Rates are charged in 1/4 hour increments according to the rate structure below
- A setup charge occurs with each use (see setup times below), therefore it is advantageous to batch your use if at all possible.
- Bills are sent to the principle investigator quarterly and are billed to the PI's grant number. Please advise us of changes in funding status or desire to bill another account.
Instrument Setup Times
FACScan
Analyzer - Flat 0.25 hours
FACS Vantage Cell Sorter
- Time on the instruments maybe scheduled via phone or the internet once the facility has been used by a particular investigator. See below. ALL USE MUST BE SCHEDULED IN ADVANCE.
- Initial use of the instruments can be scheduled only after a required first time use consultative appointment has been made. This appointment is free and is designed to ensure that FACS analysis/sorting will meet the anticipated needs of the investigator and that there will be minimal technical problems with regards to the FACS end of the project.
- There is no penalty for canceling an appointment. However, "no shows" are charged a minimum of one hour service charge.
To schedule via internet - Enter Investigator name, date of request, and estimated number of samples to be analyzed. All efforts will be made to accommodate your request. The more advance notice that is given helps ensure that your requested time will be available.
Links to other
Flow Cytometry Core Facilities
Hospital
for Special Surgery
University of Florida
Dartmouth - Mary Hitchcock
Memorial Hospital
Roswell
Park Cancer Institute - Flow Cytometry
mgolightly@notes.cc.sunysb.edu
This page maintained
and updated by Marc Golightly,Ph.D. Last updated
April 30, 2004
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